Journal: Cell death and differentiation
Article Title: CEND1 deficiency induces mitochondrial dysfunction and cognitive impairment in Alzheimer's disease.
doi: 10.1038/s41418-022-01027-7
Figure Lengend Snippet: Fig. 3 Downregulation of CEND1 in AD mice brain and cell lines. A Hippocampus of APP/PS1 mice at the age of 10-month-old and age- matched controls were subjected to Western blotting analysis for CEND1, APP with β-actin as the loading control. mean ± SEM, n = 4 per sample, **p < 0.01, by two-tailed Student’s t-test. B, C Hippocampus lysates of 5xFAD mice at the age of 2, 4, 6, 9 month and age-matched controls were subjected to western blotting analysis for CEND1, APP with β-actin as control. mean ± SEM, n ≥4 per group, *p < 0.05,**p < 0.01, by two-tailed Student’s t-test. D Mature primary cortical neurons were treated with 10 μM Aβ(1–42) for 24 h. Protein lysates were subjected to Western blotting for CEND1, p35 and p25. mean ± SEM, n = 3 per group, **p < 0.01, by two-tailed Student’s t-test. E N2a and N2a-APP695 cells were subjected to western blotting analysis for CEND1 and APP. β-actin was used as the internal control. mean ± SEM, n = 3 per group, ***p < 0.001, by two-tailed Student’s t-test. F RT-PCR analysis of 9-month-old 5xFAD mice hippocampus for CEND1. mean ± SEM, n = 3 per group, *p < 0.05, by two-tailed Student’s t-test. G 5xFAD and control primary cortical neurons were treated with 8 nM MK8931 for 12 h. Protein lysates were subjected to Western blotting for CEND1, APP and β-actin. mean ± SEM, n = 3 per group, *p < 0.001, ***p < 0.001, by ANOVA with Dunnett’s test for post-hoc analysis.
Article Snippet: MK8931, CQ, MG132 and Mdivi-1 were from MCE.
Techniques: Western Blot, Control, Two Tailed Test, Reverse Transcription Polymerase Chain Reaction
